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Bio II Sek 1
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Rules and wisdoms for molecular biology.
How to solve a problem?
Some pictures? NEW
- We are most deeply involved in cancer research. Otherwise there would be no money.
- We do not work with transgenic organisms or radioactivity. This is too dangerous.
- The usage of the electroporation apparatus is officially prohibited
- Either it works or not. No matter how. (Schiffmann, 1994)
- As long as the DNA mini prep is not restricted, you do not know wether it is the right clone or not.When you lost the DNA before restriction it was the right clone. (Schrödinger, 1981)
- It is very easy to prepare large amounts of noneRNA. (Uhrig, 1994)
- If you are able to sequence your DNA with your primer of choice, this does not mean that you can amplify it with this primer by PCR. (Haverkamp, 1994)
- PCR-products show always the right size and can be restricted in the predicted manner. Nevertheless, something different was amplified. (Schiffmann, 1995)
- If the sequencing reaction is OK your seperating gel is miserable. If your gel is OK the reaction is shit. (Brühl, 1995, Murphy et. al, 1978)
- If something is very important it will go wrong. Even if it worked 100 times before. (Murphy et. al, 1978)
- Back again: "Blot for Schrott!" (blot for trash) Starring: Annette Wiesmann, 1998.
